P3_COMMENT=The SEQUENCE_PRIMER and SEQUENCE_PRIMER_REVCOMP are reverse compliments of each other, and each is found at two positions in the template. The causes there to be 4 pairs to be considered, including one in which the left primer it to the right of the right primer.
SEQUENCE_TEMPLATE=AGGCTAGGCGAGCTGAAAAATCCTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGCTAGGCGAGCTGAAAAATCCTAC
PRIMER_MAX_TEMPLATE_MISPRIMING=12
PRIMER_PICK_ANYWAY=1
PRIMER_PRODUCT_SIZE_RANGE=30-100
PRIMER_GC_CLAMP=0
PRIMER_FIRST_BASE_INDEX=1
PRIMER_MIN_SIZE=19
PRIMER_TASK=pick_pcr_primers
PRIMER_MIN_GC=18
PRIMER_MAX_NS_ACCEPTED=1
PRIMER_MIN_TM=50
PRIMER_EXPLAIN_FLAG=1
PRIMER_OPT_SIZE=20
PRIMER_NUM_RETURN=1
PRIMER_MAX_SIZE=27
PRIMER_TM_FORMULA=1
SEQUENCE_PRIMER=AGGCTAGGCGAGCTGAAAAATCCTAC
SEQUENCE_PRIMER_REVCOMP=GTAGGATTTTTCAGCTCGCCTAGCCT
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_THERMODYNAMIC_PARAMETERS_PATH=./primer3_config/
PRIMER_SALT_CORRECTIONS=1
PRIMER_MAX_HAIRPIN_TH=47
PRIMER_MAX_SELF_ANY_TH=47
PRIMER_MAX_SELF_END_TH=47
PRIMER_PAIR_MAX_COMPL_ANY_TH=30
PRIMER_WARNING=More than one position in template for input oligo AGGCTAGGCGAGCTGAAAAATCCTAC; More than one position in template for input oligo GTAGGATTTTTCAGCTCGCCTAGCCT
PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 4, high any compl 3, high end compl 3, left primer to right of right primer 1, ok 3
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=14.595379
PRIMER_LEFT_0_PENALTY=7.297689
PRIMER_RIGHT_0_PENALTY=7.297689
PRIMER_LEFT_0_PROBLEMS= Hairpin stability too high;
PRIMER_RIGHT_0_PROBLEMS= Hairpin stability too high;
PRIMER_LEFT_0_SEQUENCE=AGGCTAGGCGAGCTGAAAAATCCTAC
PRIMER_RIGHT_0_SEQUENCE=GTAGGATTTTTCAGCTCGCCTAGCCT
PRIMER_LEFT_0=175,26
PRIMER_RIGHT_0=200,26
PRIMER_LEFT_0_TM=58.702
PRIMER_RIGHT_0_TM=58.702
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY_TH=15.46
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=47.75
PRIMER_RIGHT_0_HAIRPIN_TH=50.05
PRIMER_LEFT_0_END_STABILITY=3.1800
PRIMER_RIGHT_0_END_STABILITY=4.5800
PRIMER_PAIR_0_COMPL_ANY_TH=58.65
PRIMER_PAIR_0_COMPL_END_TH=58.65
PRIMER_PAIR_0_PRODUCT_SIZE=26
=
